Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOXK2

Cell type

Cell type Class
Gonad
Cell type
OVCAR-5
Primary Tissue
Ovary
Site of Extraction
Ascites
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
ascites
cell line
OVCAR-5
antibody
FOXK2
cell type
ovarian cancer cell
tumor stage
high grade serous
passage
4-6

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin in cells was crosslinked with 1% paraformaldehyde and fragmented to an average size of approximately 300-500 bp by sonication. 10 μg chromatin and 5 µg antibodies were proceeded to immunoprecipitation. ChIP-seq libraries were prepared by using KAPA Hyper Prep kit (Roche Sequencing) with KAPA UDI Adaptor (Roche Sequencing).

Sequencing Platform

instrument_model
Illumina HiSeq 4000

hg38

Number of total reads
78414404
Reads aligned (%)
98.5
Duplicates removed (%)
21.9
Number of peaks
17125 (qval < 1E-05)

hg19

Number of total reads
78414404
Reads aligned (%)
97.9
Duplicates removed (%)
23.1
Number of peaks
17266 (qval < 1E-05)

Base call quality data from DBCLS SRA