GSM5278106: OVCAR-5 FOXK2 ChIP-seq rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
FOXK2
Cell type
Cell type Class
Gonad
Cell type
OVCAR-5
Primary Tissue
Ovary
Site of Extraction
Ascites
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
ascites
cell line
OVCAR-5
antibody
FOXK2
cell type
ovarian cancer cell
tumor stage
high grade serous
passage
4-6
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin in cells was crosslinked with 1% paraformaldehyde and fragmented to an average size of approximately 300-500 bp by sonication. 10 μg chromatin and 5 µg antibodies were proceeded to immunoprecipitation. ChIP-seq libraries were prepared by using KAPA Hyper Prep kit (Roche Sequencing) with KAPA UDI Adaptor (Roche Sequencing).